Observations on early development of the murine fetal oral vestibule.

نویسندگان

  • J G Kim
  • Y Iwailiao
  • Y Higashi
چکیده

Morphological and immuno/histochemical studies were performed on the vestibular lamina (VL) of gestational day 13 murine fetuses, using light microscopy (LM), confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM), in an effort to elucidate the early development of the oral vestibule. Histochemistry employing LM demonstrated some PAS-positive glycogen particles in embryonic cells of the VL, dental lamina (DL), the primary epithelial band connecting the VL and DL, and the related stomodaeal simple epithelium. On the other hand, Gomori's aldehyde fuchsine method stained certain cystine-containing intracellular granules and intercellular amorphous substances, particularly in the central VL. Intense immunoreactivity for CK-10 intermediate-sized filament proteins was demonstrated in suprabasal and superficial cells of the VL stratified keratinized epithelium. Conversely, reactions for CK-19 filaments were found diffusely in both VL and DL cells retaining the cytokeratin characteristic of the simple epithelium. TEM of the VL revealed an increment in keratinosomes, tonofilaments and desmosomes in the suprabasal layers shifting toward superficial flat parakeratinized cells. The TUNEL method using CLSM detected programmed cell death in the VL, while TEM provided no morphological evidence of necrosis or typical apoptotic features during VL development. The present results indicate that physiological (naturally occurring) cell death and exfoliation of the oral-gingival type multilayered keratinizing epithelium are essential for degeneration and separation of the VL, ultimately leading to formation of the oral vestibule.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Is there association between severe early childhood caries and weight at 25-28 ‎weeks of fetal life? A longitudinal pilot study

BACKGROUND AND AIM: Sever early dental caries in childhood is one of the health problems. This study was performed to determine the association between weight at 25 and 28 weeks of fetal life and severe early childhood caries (S-ECC) at 1.5-2 years of age among some of children in Kerman, Iran.METHODS: In this longitudinal study, 100 pregnant mothers and their newborns examined via sonography f...

متن کامل

Early oleate deficiency leads to severe defects in fetal rat liver development

Objective(s): Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat ...

متن کامل

A narrative review on fasting of pregnant women in the holy month of Ramadan

One of the basic rituals of Islam is fasting during Ramadan. In this month, all able-bodied Muslims abstain from food, fluids, smoking, and oral medications from sunrise to sunset. According to the Quran, if fasting poses major health risks to individuals, fasting during this holy month is not allowed. This study aimed to evaluate the effects of fasting on maternal and fetal health. Therefore, ...

متن کامل

One Odontogenic Cell-Population Contributes to the Development of the Mouse Incisors and of the Oral Vestibule

The area of the oral vestibule is often a place where pathologies appear (e.g., peripheral odontomas). The origin of these pathologies is not fully understood. In the present study, we traced a cell population expressing Sonic hedgehog (Shh) from the beginning of tooth development using Cre-LoxP system in the lower jaw of wild-type (WT) mice. We focused on Shh expression in the area of the earl...

متن کامل

Hyalinizing Clear Cell Carcinoma of Buccal Vestibule: A Rare Case

Hyalinizying clear cell carcinoma is a rare tumor which affects women more than men and is more common in minor salivary glands. The purpose of this paper was to describe a case of hyalinizing clear cell carcinoma of buccal vestibule in a 55-year-old woman. It is important to differentiate this neoplasm from other tumors with clear cell features because of their differences in treatment and cli...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Okajimas folia anatomica Japonica

دوره 75 2-3  شماره 

صفحات  -

تاریخ انتشار 1998